Introduction

Welcome

Welcome to the Day 1 of the R-Mind workshop. We are very pleased to host this workshop for you.

Briefly introduce yourself

  • Name
  • Position (Master’s student, PhD student, Postdoc, …)
  • Expectations for this course
  • Something special about you so that we can remember you by!

Let’s chat a bit about OMICs…

  • What is “OMICs”?
  • What is a genome?
  • How big is the human genome (in basepairs)?
    • Around 3 billion bp (3Gbp)
  • How many genes are there in the human genome?
    • GENCODE v44 | Total genes=62,700 | Protein-coding genes = 19,396
  • How about mouse genes?
    • GENCODE M33 | Total genes=56,884 | Protein-coding genes = 21,403
  • What is a transcriptome?
  • What is High-throughput Sequencing (HTS) and Next-generation sequencing (NGS)?
  • What are the most common HTS applications (i.e. types of sequencing)?
  • What is R, and why should I care?

Additional resources

Training courses: Bioinformatics Training at the Harvard Chan Bioinformatics Core
Book: Modern Statistics for Modern Biology

CheatSheets

Base R
ggplot2
dplyr

References

This workshop is based on the training materials from the Harvard Chan Bioinformatics Core (HBC).

Meeta Mistry, Mary Piper, Jihe Liu, & Radhika Khetani. (2021, May 24). hbctraining/DGE_workshop_salmon_online: Differential Gene Expression Workshop Lessons from HCBC (first release). Zenodo. https://doi.org/10.5281/zenodo.4783481